Hygromycin B Powder – www.backstage-avocats.fr

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Hygromycin B Powder

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Description

Hygromycin B PowderEmpirical Formula (Hill Notation): C20H37N3O13 Molecular Weight = 527. 5 Hygromycin B is an aminoglycoside produced by Streptomyces hygroscopicus. The antibiotic interferes with the translation step of polypeptide synthesis of prokaryotes and eukaryotes. The antibiotic inhibits peptide chain elongation by preventing elongation factor EF 2 dependent translocation. Hygromycin B is used as a selective agent in yeasts, bacterial and plant cells. The

The FM drop-out mixture

The cell membranes are discarded

Concentrations of Cysteine

2-(N-Morpholino) Ethanesulfonic acid C6H13NO4S·H2O=213

Peptone 5 Beef Extract 1 Yeast Extract 2 NaCl 5 Final pH 7

01 Suspend 26

2 at 25°C Suspend 60 gram powdered medium in 1 litre distilled water

It provides a very consistent composition reducing separation of components and clumping

23 pH (100 g/l) 5

The addition of a carbon source is required

2 Agar 15 Final pH 7

2% Purity (TLC) >99% Water KF 0

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